Volume 5, Issue 1 (2025)

Prevalence of Extended-Spectrum β-Lactamase-, AmpC β-Lactamase-, and Carbapenemase-Producing Escherichia coli in Fecal Samples of Stray Cats in Italy

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Written by André M. Coelho Published on Vol 5 Issue 1, 2025

Carbapenem-resistant bacteria pose a serious worldwide public health problem, and growing evidence suggests that companion animals may contribute to the expansion of antimicrobial resistance. This research assessed the intestinal presence of Escherichia coli producing extended-spectrum β-lactamase (ESBL), AmpC, and carbapenemase (CP) enzymes, along with possible associated factors, in both healthy and diseased stray cats admitted to the Veterinary Teaching Hospital of Lodi, University of Milan,

Prevalence of Staphylococcus aureus, Methicillin-Resistant Staphylococci, and Mammaliicocci in Bulk Tank Milk from Dairy Herds in a Livestock-Intensive Region of Northern Italy

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Written by Maja Steinbach Published on Vol 5 Issue 1, 2025

Staphylococcus aureus is recognized as the primary pathogen responsible for contagious mastitis in dairy cattle, whereas non-aureus staphylococci and mammaliicocci (NASM) represent the bacterial group most frequently recovered from milk samples. Apart from their role in animal disease, NASM are known to possess antimicrobial resistance (AMR) determinants that can potentially be exchanged bidirectionally with S. aureus. The occurrence of methicillin-resistant (MR) staphylococci (MRS) poses notabl

Investigation of Subclinical Endometritis and Oviductal Occlusion in Holstein Repeat Breeders: Prevalence, Risks, and Therapeutic Interventions

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Written by Jacek P Grodzicki Published on Vol 5 Issue 1, 2025

Because Repeat Breeder (RB) syndrome leads to considerable reproductive and financial losses in dairy farming, this research aimed to estimate how frequently subclinical endometritis (SE) and oviductal occlusion (OO) occur in RB cows, identify their risk factors, and explore possible treatments. Ninety-nine RB cows were examined using endometrial cytology to detect SE, diagnosed when polymorphonuclear neutrophils exceeded 5%. Oviduct patency was evaluated with the phenolsulfonphthalein (PSP) tes

Comparative Analysis of Gut Microbiota in Krškopolje Pigs Reared Under Distinct Organic Farming Systems

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Written by Ina Kayser Published on Vol 5 Issue 1, 2025

Previous studies have revealed that both the production environment (conventional versus pasture-based) and the co-housing of pigs with other farm species can alter gut microbial communities. The present research involved two separate experiments aimed at profiling the 16S fecal microbiota of the native Krškopolje pig breed. Each experiment included three treatment categories: (i) pigs raised on pasture together with small ruminants (group L1, farm 1), (ii) pigs kept indoors without any contact

High-Dietary Laminaria digitata Effects on Muscle Proteome and Metabolome in Post-Weaning Piglets

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Written by Maria Pia Ferraz Published on Vol 5 Issue 1, 2025

The brown seaweed Laminaria digitata, recognized for its prebiotic properties, combined with alginate lyase supplementation, may support the growth and muscle development of piglets during the sensitive post-weaning stage. This research investigated the impact of including 10% L. digitata and 0.01% alginate lyase on the proteomic and metabolomic profile of the longissimus lumborum in weaned piglets. The results revealed that alginate lyase produced minimal changes in muscle protein composition r

Metabolome Alterations in Wild Boars Affected by Sarcoptic Mange

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Written by Domenico Prisa Published on Vol 5 Issue 1, 2025

Sarcoptic mange is an extremely infectious parasitic skin disorder that continues to be a serious health challenge for both humans and other mammals around the globe. The disease is produced by the mite Sarcoptes scabiei, and its clinical appearance can differ widely in form and severity. Besides visible skin irritation, affected individuals often undergo disruptions in metabolism, immune defense, reproduction, body temperature control, and overall physical condition. Despite this, the full meta

Role of EtSERPIN1 Interaction with Chicken ANXA2 in Eimeria tenella Adhesion and Invasion

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Written by Jorgelina Cussa Published on Vol 5 Issue 1, 2025

Serpin family protease inhibitors (SERPINs) in protozoans are key regulators of multiple biological functions, including the entry of parasites into host cells. Despite their importance, the molecular mechanisms by which SERPINs facilitate host invasion remain largely unresolved. Here, we demonstrate that Eimeria tenella SERPIN1 (EtSERPIN1), present on the sporozoite surface, plays a functional role in cell adhesion and invasion. To identify the host membrane component mediating EtSERPIN1-driven

Initial Development of Microfluidic Platforms for Swine Sperm Sorting: Material Selection, Perfusion Mechanisms, and Flow Dynamics

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Written by Zhaobei Cai Published on Vol 5 Issue 1, 2025

Enhancing the efficiency of assisted reproductive technologies (ART) in livestock breeding largely depends on improving semen quality by isolating only the most viable spermatozoa from ejaculates. While microfluidic systems have been widely explored for sperm sorting in humans, similar investigations in boar sperm remain scarce. Moreover, although sperm cells are known to be extremely sensitive to microplastics, the potential cytotoxicity of materials employed in microfluidic chip construction h

Performance Validation of Dual Multiplex Lateral Flow Devices for Fast Screening and Genotyping of FMDV

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Written by So-Hyun Son Published on Vol 5 Issue 1, 2025

Lateral Flow Devices (LFDs) provide a rapid and practical method for detecting foot-and-mouth disease (FMD), which is particularly valuable in endemic regions with limited laboratory infrastructure. Previous prototypes allowed simple FMD confirmation, but their capacity to identify specific serotypes—critical for understanding disease spread and guiding vaccine strategies—was limited. This work reports on the validation of two multiplex LFDs constructed using well-characterized monoclonal antibo

Volume 5, Issue 1 (2025)

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