Echinococcosis is a zoonotic infection induced by Echinococcus species and is among the most significant helminthic diseases worldwide. Surgical intervention remains the preferred treatment for cystic echinococcosis. Numerous sporicidal substances have been applied to deactivate components within hydatid cysts; however, many of them trigger inflammation and adverse reactions, limiting their clinical use. This study investigated the scolicidal activity of methanolic extract from Vitis vinifera leaves against Echinococcus eggs and protoscolices, aiming to identify the optimal concentration. Protoscolices mortality and viability were examined following exposure to four V. vinifera leaf extract (VVLE) concentrations (5, 10, 30, and 50 mg/mL) for 5, 10, 20, and 30 minutes, while eggs were treated with 100, 200, and 300 mg/mL concentrations for 24 and 48 hours. Infrared spectroscopy was used to identify the major bioactive compounds within the extract. Viability testing was performed with 0.1% eosin staining. The grape leaf extract produced 100%, 91%, 60%, and 41% mortality rates after 30 minutes at 50, 30, 10, and 5 mg/mL, respectively, and caused 11% and 19% egg mortality after 24 and 48 hours at 200 mg/mL. Increasing the concentration and exposure time generally enhanced mortality. These findings indicate that V. vinifera has strong in vitro sporicidal potential. Further research is necessary to isolate the active compound, clarify its mechanism of action, and confirm efficacy through in vivo testing.
