This study aimed to characterize ovarian follicular activity and circulating hormonal patterns in Barbari goats following a single, randomly timed injection of PGF₂α during the breeding season. Ovarian structures were monitored every 12 hours using B-mode ultrasonography, while blood samples for estradiol-17β and progesterone measurement were collected at the same interval. Detection of standing estrus was performed every 6 hours using apron-fitted bucks. After PGF₂α administration, the onset of standing estrus and the time of ovulation differed significantly (p < 0.05) among early- (n = 7), intermediate- (n = 6), and late-responding (n = 6) goats. Peak estradiol-17β concentrations occurred 12 hours before ovulation. No significant differences (p > 0.05) were noted among goats in ovulatory follicle diameter or duration of standing estrus. Regression of the corpus luteum was faster (p < 0.05) in early responders compared with intermediate and late responders. Dominant follicle size and estradiol-17β concentrations varied (p < 0.05) among the groups. Although overall progesterone levels showed no significant group differences (p = 0.065), temporal changes in progesterone concentrations varied significantly (p < 0.05) across goats. These findings indicate that the timing of estrus onset, estrus cessation, and ovulation after PGF₂α administration may differ among Barbari goats due to variations in follicular development and hormone dynamics during the luteal phase.
