The emergence of Methicillin-Resistant Staphylococcus aureus (MRSA) strains has motivated this research to explore alternative treatment options. This study focuses on evaluating the combined antibacterial activity of ethanol extracts from Persea americana and Psidium guajava leaves against MRSA isolates. The plant materials were powdered and extracted by cold maceration method using 80% v/v ethanol. Phytochemical analysis followed standard protocols. The antibacterial properties of the individual extracts, as well as their combined effect, were evaluated using the agar diffusion method. The ethanol extracts of P. americana and P. guajava were tested separately and their combined antibacterial activity was evaluated against MRSA isolates based on the inhibition zones measured using the agar cup diffusion technique. The results showed diverse inhibition zones and minimum inhibitory concentrations (MIC) for the ethanol extracts of both plants. P. americana extracts showed inhibition zones between 4 mm and 12 mm, with a MIC ranging from 50 mg/ml to 200 mg/ml. P. guajava extracts showed inhibition zones ranging from 4 mm to 12 mm, with a MIC ranging from 12.5 mg/ml to 200 mg/ml. The combined ethanol extracts of both plants showed a synergistic antibacterial effect with inhibition zones ranging from 20 millimeters to 30 millimeters. In conclusion, the combined extracts exhibited a potent antibacterial activity, indicating their potential as effective candidates to combat MRSA and help prevent drug resistance.
