Wound repair is a natural physiological process that proceeds through multiple regulated stages, including inflammation, proliferation, and tissue remodeling. For hundreds of years, Helix aspersa Muller mucus has been recognized for its beneficial biological effects in managing various skin ailments. In this research, a full-thickness excisional wound model in mice was employed to assess whether Snail Secretion Filtrate (SSF) enhances wound repair efficiency. Mucus was extracted from Helix aspersa Muller through gentle mechanical stimulation using a sterile cotton swab and subsequently purified via a series of filtrations to yield SSF. After injury induction, mice received topical SSF treatment daily for 14 days. Macroscopic analysis revealed that SSF markedly accelerated wound closure and increased the percentage of healed tissue area. Moreover, SSF promoted essential wound repair markers such as collagen formation (Masson, COL3A1, and matrix metalloproteinases (MMPs)) and supported tissue remodeling (α-sma, vascular endothelial growth factor (VEGF)). Additionally, SSF mitigated inflammatory activity in damaged tissue (myeloperoxidase (MPO), IL-1β, IL-6, TNF-α). In summary, SSF improved both the rate and quality of wound recovery by modulating several biological phases, particularly the proliferative and remodeling stages.
